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Analysis of Alkaline Phosphatase Activity
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After 3, 6, 9, and 12 days of cell culture, the activity of ALP of osteoblasts was measured by Para-nitrophenyl phosphate (pNPP). The culture medium was removed, and 0.5% Triton X-100 cell lysate (50 μl) was added at 4°C for 1 h. The 20 μl lysate was taken on a 96-well plate and operated according to the operation table of the Alkaline phosphatase Assay Kit. The OD value was determined by enzyme labeling instrument at 405 nm wavelength. According to the OD value of the sample, the activity value of ALP (U/L) was read on the ALP standard curve.
Copyright and License information: ©2021 Li, Zhou, Hu, Yang, Ye, Zhou, Li, Chen and Zhou.
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