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WGA staining and immunofluorescence

SS Shu-Ning Sun
SN Shi-Hao Ni
YL Yue Li
XL Xin Liu
JD Jian-Ping Deng
ZC Zi-Xin Chen
HL Huan Li
WF Wen-Jun Feng
YH Yu-Sheng Huang
DL Da-Nian Li
SX Shao-Xiang Xian
ZY Zhong-Qi Yang
LW Ling-Jun Wang
LL Lu Lu
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Hearts were harvested and fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into sections. The sections were dewaxed and incubated in water at 80 °C for 20 min. After cooling, the sections were washed, stained with wheat germ agglutinin (WGA), and subsequently incubated at 37 °C for 10 min. After the section were washed, they were sealed with glycerol and finally observed and imaged.

For immunofluorescence, the heart tissue was fixed, embedded, and sliced as described above. After the tissue sections were washed, they were incubated with anti-collagen I (1:200, Affinity, Cat: AF0134), anti-α-SMA (1:200, Affinity, Cat: BF9212), and anti-osteopontin (OPN) (1:200, Affinity, Cat: BF0002) primary antibodies and a fluorescently labeled secondary antibody before being observed and imaged.

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