Human monocyte isolation and treatment

FY Fan Yang
ZH Zhenqiang He
HD Hao Duan
DZ Duo Zhang
JL Juehui Li
HY Huijuan Yang
JD Jay F. Dorsey
WZ Wei Zou
SN S. Ali Nabavizadeh
SB Stephen J. Bagley
KA Kalil Abdullah
SB Steven Brem
LZ Lin Zhang
XX Xiaowei Xu
KB Katelyn T. Byrne
RV Robert H. Vonderheide
YG Yanqing Gong
YF Yi Fan
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Primary human monocytes were provided by Human Immunology Core at the University of Pennsylvania. Peripheral blood mononuclear cells were collected from healthy human volunteer donors and monocytes were isolated following leukapheresis by negative selection. All specimens were collected under a University of Pennsylvania Institutional Review Board-approved protocol and written informed consent was obtained from each donor. We have complied with all relevant ethical regulations for work with human participants. Cells were incubated in RPMI-1640 medium supplemented with 5% fetal bovine serum (FBS) and treated with 10 ng/ml human CSF-1 (BioLegend, 574806) and 100 ng/ml human IL-6 (BioLegend, 570808).

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