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Lactate Production and Glucose Consumption
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HepG2 cells were cultured for 24 h following treatment with hsa-miR-183-5p inhibitor, inhibitor NC, hsa-miR-183-5p mimics and mimics NC. The supernatant was then harvested for the measurement of lactate production and glucose consumption. Lactate or glucose levels were quantified, using the Lactate Assay kit (JianCheng Bioengineering Institute, Nanjing, China) or the Glucose Assay kit (Rongsheng Biotech, Shanghai, China), respectively, according to the manufacturer’s instructions.
Copyright and License information: ©2021 Niu et al.
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