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All statistical analysis were performed with the SPSS software v.22 (IBM Corp., Armonk, NY, USA). Kolmogorov-Smirnov test was performed to assess of normality of data. Qualitative changes were evaluated by Chi Square and Cramer's V (significance V > 0.3). For non-parametric data, the Man-Withney or Kruskal-Wallys test was used to detect significant differences between groups (p < 0.001). Normal data were compared with no paired Student t or ANOVA test. Scheffe test was used for multiple comparison data post-hoc. P < 0.05 was considered as significant.
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