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Synthesis of DexVS

JL Jifeng Liu
HL Hongyan Long
DZ Dagmar Zeuschner
AR Andreas F. B. Räder
WP William J. Polacheck
HK Horst Kessler
LS Lydia Sorokin
BT Britta Trappmann
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Vinyl sulfone functionalized dextran (MP Biomedicals, MW 86,000 Da) was prepared following a modified literature protocol51. In brief, divinyl sulfone (4.96 mL) was added to an aqueous solution of sodium hydroxide (0.1 M, 200 mL) and dextran (4 g) under vigorous stirring at room temperature. After 5 min, the reaction was stopped by the addition of hydrochloric acid to adjust the pH of the solution to 5. The crude product was then purified by dialysis (SnakeSkin™ Dialysis Tubing, Life Technologies, 10 kDa MW cutoff) against Milli-Q water for 3 days, with two water changes daily and the final product was recovered by lyophilization. 1H-NMR analysis in D2O revealed a vinyl sulfone/dextran repeat unit ratio of 0.63.

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