Fresh guava leaves were collected from the guava plantations in Qionghai City of Hainan Province, China, during the pruning period in July 2018, and fresh leaves were dried (60°C, 24 h) and powdered. The powder (50 kg) was exhaustively extracted with 95% ethanol three times at room temperature and then filtered. The solvent was evaporated under reduced pressure using a rotary vacuum evaporator to afford GE (3.47 kg), which was stored at 4°C for an animal experiment.
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