Chou-Talalay Analysis

Pharmacological interaction between Z_HPV16E61235 and Z_HPV16E7384 was determined using Chou-Talalay analysis (Chou, 2010). Briefly, 5 × 10³ cells were seeded onto 96-well plates and subsequently incubated for 2 days with the indicated agents at different concentration combinations. Then, a CCK8 assay was used to measure the combined effect of the two therapeutic agents from different groups on TC-1 and CaSki cells. Using Compusyn software², dose-effect curves for each regimen and for the combination of agents were plotted and an estimate of the combination index (CI) is achieved. A CI of >1, <1, = 1 denotes antagonism, synergy, and additive effects, respectively.