Clonogenic Assays

CW Chun-Te Wu
YH Yun-Ching Huang
WC Wen-Cheng Chen
MC Miao-Fen Chen
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To determine intrinsic cellular radiosensitivities, clonogenic assays were performed. Exponentially growing cells were irradiated and incubated for 10 days at 37°C. The plates were stained with crystal violet (Sigma) to aid colony counting. Colonies containing >50 cells were scored to determine plating efficiency and the fractions of the cells surviving after application of the various treatments.

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