Cells were treated with cisplatin for 5 h, before DNA was extracted. Platinum adduct levels were measured by ICP-MS using an Agilent 7900 mass spectrometer equipped with a micromist nebulizer and a cooled spray chamber (Agilent Technologies, USA). The assay was calibrated using platinum standards (Specure®, Alfa Aesar, Ward Hill, MA) and quality control samples with known platinum concentrations (ClinChek® Serum Controls, RECIPE, Munich, Germany). Platinum adducts were measured as previously described [50] in no-gas mode. Each measurement was made at 6 points on the mass peak in triplicate.
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