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Knockdown of SMARCA4 gene expression using siRNA treatment

HC Hae In Choi
GA Ga Yeong An
MB Mina Baek
EY Eunyoung Yoo
JC Jin Choul Chai
YL Young Seek Lee
KJ Kyoung Hwa Jung
YC Young Gyu Chai
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Knockdown (KD) of gene expression was performed using small interfering RNA (siRNA). After seeding the cells, transfection was performed using Lipofectamine RNAiMax (Invitrogen, CA, USA) transfection agent according to the manufacturer’s instructions with siRNA constructs and scrambled siRNAs. SMARCA4 siRNA (ID numbers 4677; 5′-GCAUUUCAAGGAAUAUCACtt-3′) and Silencer Negative Control siRNA (AM4611) were purchased from Thermo Fisher Scientific. SMARCA4 siRNA and scrambled siRNA were used at a 10 nM concentration for 48 h in a growth medium.

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