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Lactate Dehydrogenase (LDH) Release Assay

YS Yi Sun
SH Shuna Huang
CW Chunyu Wan
QR Qishuang Ruan
XX Xiaoxu Xie
DW Donghong Wei
GL Guobo Li
SL Shaowei Lin
HL Huangyuan Li
SW Siying Wu
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Supernatant of homogenized cells was used to measure LDH activity using a LDH kit (Built Bioengineering Institute, Nanjing, China) as suggested by the manufacturer. Absorbance of the solution was measured at 450 nm using a microplate reader (Thermo Scientific, MULTISKAN MK3, Beijing, China). And three replicates were performed for each group. Protein concentrations were calculated by standard controls provided in the kit.

Copyright and License information:  ©2021 Sun, Huang, Wan, Ruan, Xie, Wei, Li, Lin, Li and Wu.
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