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Cells were cytospun using Shandon Cytospin 3 (Block Scientific, Inc.) at 500 r.p.m. for 3 min. Air-dried slides were stained in May–Grünwald solution (Merck) for 5 min. After a brief wash with water, the slides were then stained in Giemsa solution (Histolabs) for 10 min. The slides were finally washed in distilled water and allowed to air dry before microscopic analysis. The number of enucleated cells/nucleated cells were manually counted from 50 to 70 fields in each cytospin slide.
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