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The viability of cortical neurons was measured by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, M2128) reduction assay, which is indicative of viable mitochondria. Cells were exposed for 2 h to GD and 22 h of GR; after 22 h of GR, MTT (60 μg/ml) was added and incubated for 1 h at 37 °C. The resulting formazan salt was dissolved with 2-propanol-HCl and monitored at 570 nm in a spectrophotometer. Data are expressed as a percent of control.
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