Protein sequence analysis for IDR

Domain architecture of each kinase was analyzed by SMART (http://smart.embl.de/). Intrinsic disorder tendency was predicted by IUPred2A (https://iupred2a.elte.hu/) with scores assigned between 0 and 1. A score above 0.5 indicates disorder. Predicted IDRs and their boundaries were marked on domain architecture. Prion-like domains (PrD.like, red) of each kinase were predicted by PLAAC (http://plaac.wi.mit.edu/) with scores assigned between 0 and 1. A score above 0.5 indicates prion-like region. FoldIndex (gray), -PLACC (red), and −4*PAPA (green) were also produced by PLAAC (http://plaac.wi.mit.edu/). FoldIndex (gray) predicts whether a given protein sequence is intrinsically unfolded with scores assigned between −1 and 1. A score above 0 indicates folded region, while a score below 0 indicates unfolded region. Sliding averages of per-residue log-likelihood ratios for the prion-like versus background state were scaled by using base −4 logarithms and reversed in sign, thereby producing -PLACC (red). A prion aggregation prediction algorithm (PAPA) focusing on amino acid composition of proteins predicts prion propensity of Q/N-rich proteins. In order to be more comparable to the other tracks, PAPA multiplied by −4 produces 4*PAPA (green), so that lower scores are more predictive of prion propensity. −4*PAPA below the dashed green line (−0.2) indicates prion propensity.