SYBR Green qPCR

All SYBR Green-based mixes are thoroughly described in Supplementary Table 1. For every reaction the qPCR cycling was done with a 95°C-15'' denaturation step and 40 cycles of 60°C-40'' annealing/elongation, followed by a melting curve. The activation time was adapted to the one recommended by the manufacturers of each mix or Taq Polymerase (Supplementary Table 1). The master mixes tested were: INBIO Highway qPCR Mix (Ref M130, https://www.inbiohw.com.ar/es/productos/178591/pcr/Master-Mix-qPCR-con-SYBR.html), Solis Biodyne 5x HOT FIREPol EvaGreen® qPCR Mix Plus and Biodynamics Real Mix Ref B124-100. Two custom-made mixes were prepared using different Taq Polymerases (INBIO Highway T-FREE Taq REF E1202, https://www.inbiohw.com.ar/es/productos/168157/pcr/Kit-T-Free-ADN-polimerasa.html, and Qiagen Hot Start Taq REF 203203) with SYBR® Green I (Sigma-Aldrich S9430).