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Determination of Cardiac Lipid Profile
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Cardiac tissue supernatants were incubated overnight (12 h) with 0.1 mM FeSO4 and 240 μg/ml of T. oblongata DCM extract or gallic acid as described above. The samples were centrifuged at 15,000 rpm for 10 min at 4°C. The supernatants were immediately assayed for lipid profile levels including, total cholesterol, triglycerides, and HDL-cholesterol using an Automated Chemistry Analyzer (Labmax Plenno, Labtest Co. Ltd., Lagoa Santa, Brazil) with commercial assay kits according to manufacturer’s manual.
Copyright and License information: ©2021 Erukainure, Chukwuma, Matsabisa, Javu, Salau, Koorbanally and Islam.
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