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On the day of the assay, the DiI-labeled tumor cells were harvested and 2x104 cells per well were plated in a 96-wells plate for the cytotoxicity assay. NK cells were harvested, washed and co-cultured with the tumor cells in 1:1 Effector : Target (E:T) ratio for 4 hours in the presence of different glucose concentrations (named ‘killing condition’) as indicated in the figures. Specific cytotoxicity was calculated as follows: (% dead tumor cells - % spontaneous tumor cell death)/(100% - % spontaneous tumor cell death) x100.
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