Rapamycin Administration

Rapamycin (LC Laboratories, Woburn, MA) was dissolved in 100% ethanol (30 mg/ml) and stored at −20°C until use. Immediately before injection, rapamycin was diluted (1:10) into vehicle (5% Tween80, 5% PEG400, 5% EtOH in 1× PBS). Moderately injured IGFtg and WT mice in a 3-day (Figure 1B) and a 10-day (Figure 1C) survival cohort were randomly assigned to either vehicle or rapamycin treatment groups for each experiment. The 3-day survival cohort received daily intraperitoneal (i.p.) injections of 10 mg/kg rapamycin (n = 3/genotype) or vehicle (n = 3/genotype) beginning 1 h after CCI. The 10-day survival cohort mice received a daily i.p. injection of 10 mg/kg rapamycin (n = 8/genotype) or vehicle (n = 6–7/genotype) beginning 1 h after the last BrdU injection.