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CCD-18Co colon fibroblast cell culture

AÓ Anna Ónody
AV Apor Veres-Székely
DP Domonkos Pap
RR Réka Rokonay
BS Beáta Szebeni
ES Erna Sziksz
FO Franz Oswald
GV Gábor Veres
ÁC Áron Cseh
AS Attila J. Szabó
ÁV Ádám Vannay
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CCD-18Co human colon fibroblast cell line (ATCC, Manassas, VA, USA) was cultured in Eagle's Minimum Essential Medium (EMEM) (ATCC) supplemented with L-glutamine, 10% heat-inactivated FBS (Invitrogen) and 1% streptomycin and penicillin (Sigma-Aldrich) at 37 °C and 5% CO2. To perform mRNA expression analysis and Annexin/PI staining, CCD-18Co cells were seeded in 6-well plates (n = 6 well/treatment group) at a density of 5 × 105 cells/well, for fibroblast migration, MTT and SiriusRed assays, cells were seeded into 96-well plates at a density of 104 cells/well (n = 5 well/treatment group). After plating, cells were treated with recombinant IL-24 (100 ng/ml, R&D), TGF-β1 (1 nM, R&D) or PDGF-B (10 ng/ml, R&D). Control cells were treated with corresponding solvents (IL-24 and PDGF-B: PBS; TGF-β1: 4 mM HCl) alone.

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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