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DCFDA/H2DCFDA Cellular ROS Assay

AA Andrew Alsterda
KA Kumari Asha
OP Olivia Powrozek
MR Miroslava Repak
SG Sudeshna Goswami
AD Alexandra M. Dunn
HM Heidi C. Memmel
NS Neelam Sharma-Walia
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DCFDA/H2DCFDA (2’,7’–dichlorofluorescin diacetate, also known as H2DCFDA) is a fluorogenic dye that measures hydroxyl, peroxyl, and other reactive oxygen species (ROS) activity within the cell. DCFDA/H2DCFDA is a cell-permeant reagent and cellular ROS assay from Abcam (#ab113851) quantitatively assess reactive oxygen species in live cell samples. Briefly, untreated Salubrinal treated, and NAC (5 mM), and Salubrinal treated cells were cultured 48h. Cells were collected and stained (30 min at 37°C) with oxidative-sensitive dye DCFDA/H2DCFDA and analyzed immediately with a flowcytometer as per the manufacturer’s instructions. Exogenous H2O2 is frequently used as a representative ROS in modeling and inducing oxidative stress.

Copyright and License information:  ©2021 Alsterda, Asha, Powrozek, Repak, Goswami, Dunn, Memmel and Sharma-Walia
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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