The questionnaire was prepared in English and translated to Amharic (local language) then translated back to English to check for consistency. All study participants were informed about the aim and importance of the study before data collection to make them fully concerned about their response. The questionnaire was pre-tested and training was given for data collectors. The collected data were checked daily for consistency and accuracy. Data collection was closely supervised by investigators. To avoid hemolysis blood collection and blood handling were conducted by following all protocols.
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