2.4. Scanning Electron Microscopy of Sugarcane Bagasse Fibers

Four different circumstances were compared: only the fiber-rich sterile medium alone (i) and kept under shaking (150 rpm) (ii), and the fiber-rich medium with the community and kept under shaking (150 rpm) during the 2nd (iii) and 20th (iv) weeks of cultivation. Both samples were thawed and recovered, then cultivated in BHB medium with sugarcane bagasse for five days. These samples were filtered and separated in the attached and free fractions. Post-fixation was performed with osmium tetroxide (OsO₄) and ethanol dehydration (99 to 10%). The fixed samples were then assembled over the support and coated with gold (20–30 mm). Two sterile mediums were used (30 °C, 150 rpm, and only at 30 °C) for negative control. Imaging was performed under the scanning electron microscope (Joel JSM6610LV) in a range of magnification from 1000× to 5000×.