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Cell migration was assessed by wound-healing assay. The cells of each group were cultured to the density of fusion state. Before the experiment, the medium was changed to serum-free medium and treated with mitomycin C (1 μg/ml) for 1 h. The cells were scratched with 200 μl pipette tip and the surface of the cells was washed once with serum-free medium to remove cell debris. Cells were then incubated in an incubator at 37 °C with 5% CO2 for 0 h and 24 h, respectively, and then photographed, and the location of the cells was recorded.
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