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The total RNA of cells was extracted using TRIzol (Takara, Shiga, Japan) and transcribed into cDNA using a Primer Script RT Kit (Takara). SYBR Green (Takara) was used for a quantitative real‐time quantitative PCR (qRT‐PCR). The primers were ordered from GeneCopoeia (Rockville, MD, USA). Relative gene expression level was calculated via .
Copyright and License information: ©2021 The Authors. published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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