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Interpretation of MIC, MPC and MSW

NF Nayara Helisandra Fedrigo
DS Danielle Rosani Shinohara
JM Josmar Mazucheli
SN Sheila Alexandra Belini Nishiyama
FC Floristher Elaine Carrara-Marroni
FM Frederico Severino Martins
PZ Peijuan Zhu
MY Mingming Yu
SS Sherwin Kenneth B. Sy
MT Maria Cristina Bronharo Tognim
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The MIC was defined as the lowest concentration of drug inhibiting the visible colony growth after 20 to 24 h of incubation45. The MIC results were interpreted according to the breakpoints established by the EUCAST and CLSI guidelines for A. baumannii strains6,26.

The MPC was defined as the lowest concentration of antimicrobial that prevented colony formation from a culture containing ≥ 109 CFU bacteria. The plates were examined after the incubation period of 24 and 48 h12.

The antimicrobial concentrations range exceeding the MIC up to the MPC was defined as the MSW, at which selective amplification of antibiotic-resistant mutants occurs52.

The MPC:MIC ratio of polymyxin B alone and combined was also calculated and expressed as the ability of each combination to select resistant mutants. Lower values of the ratio indicate a better ability to restrict the emergence of resistant mutants53.

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