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Sodium Deoxycholate (SDC) and Tetraethylammonium Bromide (TEAB) Clean Up

JO Joseph O. Ojo
JR Jon M. Reed
GC Gogce Crynen
PV Prashanthi Vallabhaneni
JE James Evans
BS Benjamin Shackleton
ME Maximillian Eisenbaum
CR Charis Ringland
AE Anastasia Edsell
MM Michael Mullan
FC Fiona Crawford
CB Corbin Bachmeier
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To remove traces of SDC and TEAB, protein samples were re-suspended in 100 ul of 1% formic acid solution and centrifuged at 15,000 rpm for 1 min to allow separation into different phases. Supernatants were collected in new Eppendorf tubes, and 200 ul of ethyl acetate was added, and centrifuged at 15,000 rpm with the upper organic layer discarded. This process was repeated three separate times, with the final lower phase taken to dryness in the speed vacuum. The resultant dried samples were re-solubilized in 100 ul of 0.1% formic acid.

Copyright and License information:  ©2021 Ojo, Reed, Crynen, Vallabhaneni, Evans, Shackleton, Eisenbaum, Ringland, Edsell, Mullan, Crawford and Bachmeier.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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