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Congo red agar method (CRA)

SM Sarita Manandhar
AS Anjana Singh
AV Ajit Varma
SP Shanti Pandey
NS Neeraj Shrivastava
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The screening of biofilm production was performed using CRA media; a qualitative method as described by Freeman et al., [27]. The medium composed of Brain Heart Infusion (BHI) agar supplemented with sucrose and a dye; Congo red. These plates were inoculated with CNS isolates, incubated aerobically at 37 ºC for 24 h and were interpreted based on the qualitative observation of colored colonies formation on the CRA plates. The black colonies with dark consistency were regarded as strong biofilm producers while the pink colonies were regarded as biofilm non-producers. The experiments were performed in triplicates and repeated three times.

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