2.5. Isolation of NG2+ cells by magnetic cell sorting

NG2+ cells were isolated using the NTDK-P Kit (Miltenyi Biotec) according to previously described procedures (Diers-Fenger et al., 2001; Hoch-Kraft et al., 2018), where Magnetic isolation (MACS) was performed with anti-NG2 antibody (Niehaus et al., 1999) conjugated with magnetic beads (Miltenyi Biotec). Cell purity and homogeneity of the sorted population was checked as shown in Supplementary Fig. 1. For the P9 time-point, each sample was a pool of the hippocampi from the male pups of the same litter; n represents litter number (control n = 7, LBN low CORT n = 8, LBN high CORT n = 7, randomized in 3 batches). For the 8-month time-point each sample was a pool of the hippocampi of 3–4 males per sample (control n = 6, LBN n = 7, randomized in 3 batches of experiments).