Tissue sections were prepared as described in immunohistochemistry. The cresyl violet solution (#G1430; Solarbio, Beijing, China) was used to stain brain sections at 56 °C for 60 min, and then rinsed briefly three times with deionised water. The samples were incubated in Nissl differentiation solution for a few seconds to 2 min, then quickly followed by dehydration with gradient alcohol as described above, cleaned with xylene I for 5 min and xylene II for 5 min, and mounted with neutral balsam. We counted and analysed the cells of Nissl staining from different groups.
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