Mice were injected intraperitoneally with 2 g/kg glucose, and ~20 µl of blood were withdrawn from tail bleeding before and at the indicated time points after injection. Serum was separated and kept at −80 °C until analysis. Insulin levels in serum samples were measured with ultra-sensitive mouse insulin ELISA kit (Crystal Chem) following the manufacturer’s instructions.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.