Glucose-stimulated insulin secretion (GSIS)

AR A. Roichman
SE S. Elhanati
MA M. A. Aon
IA I. Abramovich
AF A. Di Francesco
YS Y. Shahar
MA M. Y. Avivi
MS M. Shurgi
AR A. Rubinstein
YW Y. Wiesner
AS A. Shuchami
ZP Z. Petrover
IL I. Lebenthal-Loinger
OY O. Yaron
AL A. Lyashkov
CU C. Ubaida-Mohien
YK Y. Kanfi
BL B. Lerrer
PF P. J. Fernández-Marcos
MS M. Serrano
EG E. Gottlieb
RC R. de Cabo
HC H. Y. Cohen
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Mice were injected intraperitoneally with 2 g/kg glucose, and ~20 µl of blood were withdrawn from tail bleeding before and at the indicated time points after injection. Serum was separated and kept at −80 °C until analysis. Insulin levels in serum samples were measured with ultra-sensitive mouse insulin ELISA kit (Crystal Chem) following the manufacturer’s instructions.

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