Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

CellTiter-Glo luminescent viability assay

SA Surein Arulananda
MO Megan O’Brien
ME Marco Evangelista
LJ Laura J. Jenkins
AP Ashleigh R. Poh
MW Marzena Walkiewicz
TL Trishe Leong
JM John M. Mariadason
JC Jonathan Cebon
SB Srividya B. Balachander
JC Justin R. Cidado
EL Erinna F. Lee
TJ Thomas John
WF Walter D. Fairlie
request Request a Protocol
ask Ask a question
Favorite

Cells (1000 per well) were seeded into 96-well white plates and 4 h later treated with serial dilutions of vehicle (DMSO or DMF), drugs alone, or combinations thereof. Cell viability was assessed after 72 h treatment using the CellTiter-Glo 2.0 assay (cat# G9243, Promega, Australia) following the manufacturer’s instructions. Luminescence was measured on an Ensight Multimode plate reader (Perkin Elmer). The results were normalized to the viability of cells treated with the highest % (v/v) of vehicle. The EC50 values were calculated using non-linear regression algorithms using the Prism software (Graph Pad, version 6) from the combined data of at least three separate experiments. Synergy analysis was performed using Combenefit software with Bliss, Loewe and HSA models31.

Copyright and License information: The Author(s) ©2021
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A