Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Culture of BAECs and HEK293 cells

CL Chang Li
AH An He
YG Yongzheng Guo
XY Xiyang Yang
ML Minghao Luo
ZC Zhe Cheng
LH Longxiang Huang
YX Yong Xia
SL Suxin Luo
request Request a Protocol
ask Ask a question
Favorite

Bovine aortic endothelial cells (BAECs) and the human embryonic kidney cell line HEK293 (kindly provided by Dr. Yong Xia) were cultured with DMEM supplemented with 10% FBS and incubated in an incubator with 5% CO2 and 95% O2. BAECs from passages 6 to 10 and with 80% to 90% purity were used for experiments. Cells were randomly assigned various treatments, including hypertonic stress (130 mM, 150 mM, 170 mM, and 190 mM NaCl for 24 h), heat stress (40 °C for 2 h), oxidative stress (200 mM hydrogen peroxide for 24 h or 200 mM ethanol for 24 h), hypoxia (1% O2 for 12 h) and acidosis (pH 7, pH 6.5 for 6 h). The control group was cultured with conventional medium for the same time.

Copyright and License information: The Author(s) ©2021
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A