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SU-DHL-1 and SU-DHL-6 cells (0.5 × 106 cells/ml) were transfected with either precursor miR-1250-5p mimics or scramble oligonucleotides negative control (Ambion, Austin, TX, USA) at final concentration of 150 nM with Lipofectamine 2000 transfection reagent (Invitrogen, Carlsbad, CA, USA). The transfected cells were cultured under serum-free condition for 48–72 h.

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