4.2. Ribonucleic Acid (RNA) Extraction and Sequencing

After three passages in embryonated chicken eggs, RNA was extracted from allantoic fluid using Trizol LS^® reagent (Ambion, Invitrogen Canada Inc., Burlington, ON, Canada) according to the manufacturer’s protocol. The genome was transcribed to complimentary deoxyribonucleic acid (cDNA) using random primers (High Capacity Reverse Transcription Kit, Applied Biosystems™, Invitrogen Canada Inc., Burlington, ON, Canada) according to the manufacturer’s instructions. Whole genome sequencing on the cDNA library was performed at the Faculty of Veterinary Medicine, University of Montreal, Montreal QC, Canada, using a Miseq platform (Illumina corp, San Diego, CA, USA). The genome was deposited in GenBank under the accession number MT665806.