4.13. Passive Avoidance Test

The passive avoidance test used two equally sized compartments (17 × 12 × 10 cm) set up with an electrifiable grid floor and separated by a guillotine door. On day 1, mice were initially placed in the light compartment to acclimatize for 40 s. The door was automatically opened and closed after the mice entered the dark compartment and were given a low-intensity electric foot shock (0.5 mA, 3 s) in the dark compartment. The transference of mice from the light to dark compartments was recorded in seconds as the transfer latency time. On day 2, no foot shock was delivered, and the duration of the trial was 300 s to examine the transfer latency time. The transfer latency time was measured using the Gemini Avoidance System (San Diego Instruments, San Diego, CA, USA).