4.9. Flow Cytometry Analysis

Apoptosis was detected with an annexin V-FITC/PI detection kit. After exposure to CET (0, 5, 10, 20 μM) for 24 h, HL-60 cells were harvested and washed twice with ice-cold PBS. Then the cells were suspended in 500 μL binding buffer and incubated with 5 μL annexin V-FITC in the dark for 10 min. Next, after staining with 5 μL PI solution for 5 min, cells were analyzed by flow cytometry. Flow cytometry analysis were performed using a Kaluza Analysis 2.1 (Kaluza software, Fullerton, CA, USA).