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2.3.1. OD Measurements

RS Rana Salem
MS Moomen Soliman
AF Ahmed Fergala
GA Gerald F. Audette
AE Ahmed ElDyasti
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A periodic measurement of the optical density (OD) for monitoring the growth of the bacterial culture in both the growth and PHB accumulation phases was performed using DR 3900 Benchtop Spectrophotometer (HACH Company, Loveland, CO, USA) at (OD600). A calibration curve was created to determine the correlation equation between the volatile suspended solids (VSS) assessed following standard protocol and the OD. The VSS was further used to calculate the specific growth rate (µ) and the biomass yield (Y) using Equations (1) and (2), respectively [23].

where VSSf  is the final volatile suspended solids in mg, VSSi  is the initial volatile suspended solids in mg, DCWav. is the average of the initial and final volatile suspended solids in mg, and t is time in h.

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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