4.5.3. GC-HRMS

Following the procedure described in Hernandez-Alonso et al. [34], samples were analyzed in a 7890A Series GC coupled to a triple quadrupole (QqQ) (7000 series; Agilent Technologies, Barcelona, Spain) using the J&W Scientific HP5-MS (30 m × 0.25 mm i.d., 0.25 µm film; Agilent Technologies, Barcelona, Spain) chromatographic column and helium as a carrier gas. Ionization was carried out with electronic impact recording data in “Full Scan” mode.

Metabolite measurements were based on specific RT plus an ion fragmentation pattern. Quantification was performed by internal standard calibration, using the corresponding analytical standard for each determined metabolite (succinic d4 acid, glycerol 13C3, norvaline, L-methionine-(carboxy-13C, methyl-d3), D-glucose 13C6, myristic-d27 acid, and alpha-tocopherol d6), and a deuterated internal standard depending on the family of metabolite.