2.8. DNA Quantification and Purity

DNA concentration of EBN samples was quantified with spectrophotometric assay by measuring UV absorbance at 260 nm (A₂₆₀) using a BioSpectrometer^® kinetic spectrophotometer (Eppendorf, Hamburg, Germany). One-way analysis of variance (ANOVA) was performed to compare the DNA concentration between five different DNA extraction methods (Table 1). Significant differences between means were evaluated using Tukey’s test at a confidence level of 95%. Fluorometric quantification assay was also performed based on fluorescent DNA binding dyes using a Qubit^® 2.0 fluorometer (Life Technologies, Carlsbad, CA, USA) and Qubit^® dsDNA high sensitivity assay kit. This assay is highly specific and selective for double-stranded DNA (dsDNA) quantification. Purity of the extracted DNA was determined by the absorbance ratios of 260 and 280 nm (A₂₆₀/A₂₈₀) using the spectrophotometer.