Hematoxylin & Eosin (H&E) staining

After removing the culture media, cells were washed with PBS 2 times. 3.7% formaldehyde solution was treated to fix the samples for 15 min at room temperature (RT). After washing cells with PBS for 3 times, 0.2% Triton X-100 solution was used for permeabilization. Cells were then washed with PBS for 3 times and washed with distilled water 2 times. Hematoxylin was treated for 1 min at RT. Cells were washed with tap water to remove hematoxylin and eosin was treated for 45 s. Immersion with 95% and 100% ethanol was used to remove the eosin dye.