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4.2. PCR Genotyping and qRT-PCR for Gene Expression Analysis
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Total RNA was extracted from leaf tissue using the RNeasy mini kit (Sigma-Aldrich, St. Louis, MO, USA), and then cDNA was synthesized using Verso cDNA synthesis kit (Thermo Scientific, Waltham, MA, USA). The qRT-PCR was performed following the instructions for the Mastercycler ep realplex (Eppendorf, Hamburg, Germany), using gene-specific primers with SYBR green mix kit (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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