2.6. Cell Lines and Cultivation

Human cell cultures of tumor origin (A549 (lung carcinoma), HCT116 (colon adenocarcinoma), MCF7 (breast cancer), RD (embryonic rhabdomyosarcoma)), and the line of non-transformed cells HEK293 (embryonic kidney) provided by the Laboratory of Tumor Cell Genetics at the N.N. Blokhin Russian Cancer Research Institute, as well as by the Institute of Cytology of the Russian Academy of Sciences, were grown in DMEM (Gibco, Scotland, UK) and MEM (Gibco, Scotland, UK) containing fetal calf serum (10% by volume) (ThermoFisher Scientific, Paisley, UK), L-glutamine (2 mM) (Gibco, Scotland, UK), and penicillin-streptomycin (1% by volume) (PanEco, Moscow, Russia). The cultivation was carried out at 37 °C in a humidified CO₂ atmosphere (5%).