The sulforhodamine B colorimetric assay measures the cellular protein content, and the assay results are proportional to the number of cells. This assay has been used to determine the cell density; however, it does not distinguish between living and dead cells [33].
The SRB assay was performed using a commercially available kit (TOX6) (Sigma-Aldrich, Poland) according to the manufacturer’s protocol. In this assay, SRB binds to protein components of trichloroacetic acid-fixed cells, and the protein-bound dye is extracted with Tris. The results obtained are presented as the percentage of the total cellular protein content in control (nontreated) cells.
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