A stock solution of betulin was prepared in DMSO at a concentration of 2500 µg/mL, and working solutions were prepared in cell maintenance media (serum-free) at final concentrations of 0 (control cells), 0.244, 0.488, 0.976, 1.95, 3.9, 7.8, 15.625 and 31.25 µg/mL immediately before use in cell culture.
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