2.10. Bone Histology of Minipig Model

The samples were processed using the same procedure as that applied to the rat femurs (see Section 2.5). Sections of the pig femurs were stained using 20% Giemsa’s azur eosin methylene blue solution (Merck Millipore, Darmstadt, Germany) [50] and the whole surface of the implants photographed using a bright field microscope with a 10× objective. All the steps applied during the microscopic evaluation complied with ISO 10993-6.