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2.8. DanioVision

LB Letizia Brogi
MM Maria Marchese
AC Alessandro Cellerino
RL Rosario Licitra
VN Valentina Naef
SM Serena Mero
CB Carlo Bibbiani
BF Baldassare Fronte
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DanioVision® (Noldus©, Wageningen, Wageningen, The Netherlands) is a complete activity monitoring system for zebrafish larvae interfaced with a specific video tracking software (EthoVision XT®, Noldus©, Wageningen, The Netherlands) that allowed to measure the following parameters: (a) distance travelled (mm); (b) velocity (mm/s); (c) Movement Cumulative Time (MCT; s). MCT is the total time spent moving by larvae over the duration of the experiment. To this purpose, at 120 hpf the treated larvae were transferred into 96 multiwell plates containing 100 μL egg water per well. Hence, one plate per time was placed in the DanioVision® device and larval activity monitored for 30 min. Finally, EthoVision XT® software produced a spreadsheet data set, suitable for subsequent statistical analysis.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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