request Request a Protocol
ask Ask a question
Favorite

Using raw genera counts, normalization factors were calculated with the TMM method using the calcNormFactors function in edgeR R package v 3.32.1. Common and tagwise dispersions were then estimated using estimateCommonDisp and estimateTagwiseDisp functions in edgeR. Testing for differential relative abundance between PD and controls was performed using exactTest function in edgeR. P values were corrected for multiple testing using BH FDR method implemented in the topTags function in edgeR.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A