2.6.6. Swarming and Swimming Motility Inhibition on P. aeruginosa PA01

The inhibition of a swarming motility assay was done as described previously [18]. Briefly, overnight cultures of P. aeruginosa PAO1 strain were point-inoculated at the center of swarming plates consisting of 1% peptone, 0.5% NaCl, 0.5% agar, and 0.5% of filter-sterilized D-glucose with various concentrations of KC1-1 or KC2-1 (50, 75, and 100 µg/mL), a plate without the samples was used as control. Plates were incubated for 18 h. The swarming migration was recorded by following swarm fronts of the bacterial cells.

For swimming motility assay, the P. aeruginosa PAO1 strain was inoculated at the center of the swarming agar medium consisting of 1% peptone, 0.5% NaCl, 1.5% agar, and 0.5% of filter-sterilized D-glucose with increasing concentrations of propolis/cerumen (50, 75 and 100 μg/mL). The plates were then wrapped with Saran Wrap to prevent dehydration and incubated at 37 °C in an upright position. The reduction in swimming migration was recorded by measuring the swim zones of the cells after 16 h.