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2.3. Fatty Aldehyde Dehydrogenase Activity Assay
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Determination of FALDH activity was performed by using the same reaction mixture and detection of pyrenedecanoic acid as described for AGMO activity assessment. Instead of AGMO substrate, 30 µM of pyrene-labelled FALDH substrate pyrenedecanal (PDA) was used. Production of pyrenedecanoic acid was determined in the presence of 10 or 100 µM BH4 and 300 µM compound 6 or DMSO vehicle control.
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